Laboratory Medicine

Test catalogue

TB Quantiferon (IGRA)
Test Name TB Quantiferon (IGRA)
Specimen Type / Requirements Container Type = Specialized Tubes (QFT TB Kit) Required For Testing Call Microbiology at 5381 to obtain kit Note: Outpatient Tb Quantiferon request are done at Dynacare.
Specimen Handling Collection Instructions: prior arrangement Ensure both requisitions and samples are labelled properly. Requisition must be completed with all of the required information. Immediately after filling tubes invert tubes ten (10) times just firmly enough to ensure the entire surface of the tube is coated with blood. Over-energetic shaking may cause gel disruption and could lead to aberrant results. Write your initials, date and time of collection on the collection label or requisition. Labotatory Staff Instructions: o When IGRA is ordered through Soarian, the floor is prompted to print out the Sick Kids requisition. o The floor will then contact Phlebotomy to have IGRA collected STAT. o Phlebotomy will collect the Lithium Heparin tube. Label the tube and Sick Kids requisition with LIS labels. o Phlebotomy will deliver the sample and Sick Kids requisition to the Microbiology Lab. Tubes MUST BE received in the Sick Kids Microbiology Laboratory within 12 hours of collection. Rejection Criteria: Tubes other than those provided in the collection kit. Unlabeled or samples of non sufficient quantity. Transportation: DO NOT refrigerate or freeze samples, ship at room temperature.
Required Documentation
Turnaround Time (TAT) 2 weeks	Test Utilization
Reference Value Positive Test Result: > 0.350 IU/mL Negative Test Result: < 0.350 IU/mL Indeterminate Result: Due to high Nil value or low mitogen response
Test code MISC	Methodology ELISA
Testing Location SMH-Microbiology	Other Information Last Updated: April 27th 2021

TB Quantiferon (IGRA)

Test Name

TB Quantiferon (IGRA)

Specimen Type / Requirements

Container Type = Specialized Tubes (QFT TB Kit) Required For Testing

Call Microbiology at 5381 to obtain kit

Note: Outpatient Tb Quantiferon request are done at Dynacare.

Specimen Handling

Collection Instructions: prior arrangement Ensure both requisitions and samples are labelled properly. Requisition must be completed with all of the required information. Immediately after filling tubes invert tubes ten (10) times just firmly enough to ensure the entire surface of the tube is coated with blood. Over-energetic shaking may cause gel disruption and could lead to aberrant results.

Write your initials, date and time of collection on the collection label or requisition.

Labotatory Staff Instructions:

o When IGRA is ordered through Soarian, the floor is prompted to print out the Sick Kids requisition.

o The floor will then contact Phlebotomy to have IGRA collected STAT.

o Phlebotomy will collect the Lithium Heparin tube. Label the tube and Sick Kids requisition with LIS labels.

o Phlebotomy will deliver the sample and Sick Kids requisition to the Microbiology Lab.

Tubes MUST BE received in the Sick Kids Microbiology Laboratory within 12 hours of collection.

Rejection Criteria: Tubes other than those provided in the collection kit. Unlabeled or samples of non sufficient quantity.

Transportation: DO NOT refrigerate or freeze samples, ship at room temperature.

Required Documentation

Turnaround Time (TAT)

2 weeks

Test Utilization

Reference Value

Positive Test Result: > 0.350 IU/mL

Negative Test Result: < 0.350 IU/mL

Indeterminate Result: Due to high Nil value or low mitogen response

Test code

MISC

Methodology

ELISA

Testing Location

SMH-Microbiology

Other Information

Last Updated: April 27th 2021