Test performed at:

Placental Growth Factor (PlGF) & Soluble fms-like tyrosine kinase (sFlt-1):PlGF ratio
Test Name Placental Growth Factor (PlGF) & Soluble fms-like tyrosine kinase (sFlt-1):PlGF ratio
Specimen Type / Requirements Container Type = 1 Gold Top (SST) or Red Top tube Minimum Testing Volume = 0.7mL Serum Its is recommended to fill the tube to at least ½ or greater of its vacuum capacity.
Specimen Handling Collection Instructions: Collection Instructions: Collect specimen in gold or red top tube. Ensure the collection date and time is provided. Laboratory Staff Instructions: Separate samples from cells as soon as possible. Can withstand up to 3 freeze/thaw cycles. Rejection Criteria: Unlabeled or not sufficient quantity samples will be rejected. Stability: Stable At room temperature: 20-25°C: up to 1 day. Refrigerated at 2 8°C: up to 2 days. Frozen at -20°C or lower: up to 6 months. Transportation: Send sample to SMH core lab as soon as possible.
Downtime Documentation	Turnaround Time (TAT) 2 hours from arrival at SMH lab
Reference Value PlGF ≥ 100 pg/mL Interpretative comment: PlGF < 12 pg/mL Test positive – highly abnormal and suggestive of patients with severe placental dysfunction and at increased risk of preterm birth. PlGF 12-99 pg/mL Test positive – abnormal and suggestive of patients with placental dysfunction and at increased risk of preterm birth. PlGF ≥100 pg/mL Test negative – normal and suggestive of patients without placental dysfunction and unlikely to progress to birth within 14 days of the test. sFlt1/PlGF ratio: • ≤ 38 Low risk for PET requiring delivery within next 7 days. • 39 – 85 Moderate risk (20%) for PET within the next 7 days. Consider clinical picture as to whether to admit the patient. • >85 High Risk (56%) for PET within the next 7 days. Admit the patient and consider Celestone if < 35+0 weeks.
Test Code o LAB7601 – for PlGF only o LAB3286 – for sFLT1/PlGF Ratio	Methodology Electrochemiluminescence Immunoassay (ECLIA)
Testing Location SMH-Biochemistry	Other Information Last Updated: Aug 25, 2025

Placental Growth Factor (PlGF) & Soluble fms-like tyrosine kinase (sFlt-1):PlGF ratio

Test Name

Placental Growth Factor (PlGF) & Soluble fms-like tyrosine kinase (sFlt-1):PlGF ratio

Specimen Type / Requirements

Container Type = 1 Gold Top (SST) or Red Top tube

Minimum Testing Volume = 0.7mL Serum

Its is recommended to fill the tube to at least ½ or greater of its vacuum capacity.

Specimen Handling

Collection Instructions: Collection Instructions: Collect specimen in gold or red top tube. Ensure the collection date and time is provided.

Laboratory Staff Instructions: Separate samples from cells as soon as possible. Can withstand up to 3 freeze/thaw cycles.

Rejection Criteria: Unlabeled or not sufficient quantity samples will be rejected.

Stability: Stable At room temperature: 20-25°C: up to 1 day. Refrigerated at 2 8°C: up to 2 days. Frozen at -20°C or lower: up to 6 months.

Transportation: Send sample to SMH core lab as soon as possible.

Downtime Documentation

Turnaround Time (TAT)

2 hours from arrival at SMH lab

Reference Value

PlGF ≥ 100 pg/mL

Interpretative comment:

PlGF < 12 pg/mL Test positive – highly abnormal and suggestive of patients with severe placental dysfunction and at increased risk of preterm birth.
PlGF 12-99 pg/mL Test positive – abnormal and suggestive of patients with placental dysfunction and at increased risk of preterm birth.
PlGF ≥100 pg/mL Test negative – normal and suggestive of patients without placental dysfunction and unlikely to progress to birth within 14 days of the test.

sFlt1/PlGF ratio:

• ≤ 38 Low risk for PET requiring delivery within next 7 days.

• 39 – 85 Moderate risk (20%) for PET within the next 7 days. Consider clinical picture as to whether to admit the patient.

• >85 High Risk (56%) for PET within the next 7 days. Admit the patient and consider Celestone if < 35+0 weeks.

Test Code

o LAB7601 – for PlGF only
o LAB3286 – for sFLT1/PlGF Ratio

Methodology

Electrochemiluminescence Immunoassay (ECLIA)

Testing Location

SMH-Biochemistry

Other Information

Last Updated: Aug 25, 2025