Laboratory Medicine

Test catalogue

Jak2 Mutation (Jak2 V617F)
Test Name Jak2 Mutation ( Jak2 V617F)
Specimen Type / Requirements Container Type = Lavender Top (EDTA) Minimum Venous Volume = 3 mL whole blood
Specimen Handling Collection Instructions: Collect whole blood (3 mL) in lavender top (EDTA) tube. Do not use heparin as an anti-coagulant. Haematology consult is required for in-patient samples. If Jak2 V617F is negative, test can be Referred to UHN for Jak2 Exon-12 and Mutation of the Calreticulin (CALR) Gene. If requested, please complete this requisition and send it with sample to molecular laboratory. Write your initials, date and time of collection on the collection label. Laboratory Staff Instructions: Sample is processed at Molecular Lab. Transportation: Send sample to core lab as soon as possible. Specimens can be stored at 2-6°C for up to 7 days before DNA extraction. Sample Rejection Criteria: Clotted sample, repeat samples, unlabeled or samples with insufficient quantity will be rejected.
Required Documentation
Turnaround Time (TAT) 14 days	Test Utilization
Reference Value See report
Test Code JAK2	Methodology PCR Assay
Testing Location SMH-Molecular Diagnostic	Other Information Last Updated: June 06, 2019

Jak2 Mutation (Jak2 V617F)

Test Name

Jak2 Mutation ( Jak2 V617F)

Specimen Type / Requirements

Container Type = Lavender Top (EDTA)

Minimum Venous Volume = 3 mL whole blood

Specimen Handling

Collection Instructions: Collect whole blood (3 mL) in lavender top (EDTA) tube. Do not use heparin as an anti-coagulant. Haematology consult is required for in-patient samples. If Jak2 V617F is negative, test can be Referred to UHN for Jak2 Exon-12 and Mutation of the Calreticulin (CALR) Gene. If requested, please complete this requisition and send it with sample to molecular laboratory.

Write your initials, date and time of collection on the collection label.

Laboratory Staff Instructions: Sample is processed at Molecular Lab.

Transportation: Send sample to core lab as soon as possible. Specimens can be stored at 2-6°C for up to 7 days before DNA extraction.

Sample Rejection Criteria: Clotted sample, repeat samples, unlabeled or samples with insufficient quantity will be rejected.

Required Documentation

Turnaround Time (TAT)

14 days

Test Utilization

Reference Value

See report

Test Code

JAK2

Methodology

PCR Assay

Testing Location

SMH-Molecular Diagnostic

Other Information

Last Updated: June 06, 2019