Laboratory Medicine

Test catalogue

Heparin Induced Thrombocytopenia (HIT)
Test Name Heparin Induced Thrombocytopenia (HIT, Ant-PF4 Antibody, Heparin Induced Antibody, Anti-Heparin Platelet Antibody)
Specimen Type / Requirements Container Type = Blue Tube (Sodium Citrate) Minimum Testing Volume = Full draw 9:1 Ratio (9 parts blood to 1 part anticoagulant)
Specimen Handling Collection Instructions: Must indicate anticoagulant status to avoid delay. A Hematology consult is REQUIRED for *inpatient* samples (except for patients in Hematology/Oncology ward). *Referred-in* patients and *outpatients* can be tested without a Hematology consult. Write your initials, date and time of collection on the collection label. Transportation: In/Outpatients: Send whole citrated blood at room temperature as soon as possible. Referred-in: Send frozen citrated plasma on dry ice. All samples must be sent to the Core Laboratory. Stability: Specimens are stable for 4 hours post collection at room temperature. Specimens which are not tested within 4 hours can be double-spun to remove the citrated plasma. The citrated plasma is stable at -20°C for up to two weeks or -70°C for up to six months. Sample Rejection Criteria: Clotted sample, grossly hemolyzed samples, Unlabeled or samples with insufficient quantity will be rejected.
Required Documentation
Turnaround Time (TAT) 1 day	Test Utilization
Reference Value 0.00 – 0.60 U/mL < 1.0 U/mL is negative ≥ 1.0 U/mL is positive
Test Code HIT	Methodology Latex Immunoassay
Testing Location SMH-Special Coagulation	Other Information Last Updated: June 06, 2019

Heparin Induced Thrombocytopenia (HIT)

Test Name

Heparin Induced Thrombocytopenia (HIT, Ant-PF4 Antibody, Heparin Induced Antibody, Anti-Heparin Platelet Antibody)

Specimen Type / Requirements

Container Type = Blue Tube (Sodium Citrate)

Minimum Testing Volume = Full draw

9:1 Ratio (9 parts blood to 1 part anticoagulant)

Specimen Handling

Collection Instructions: Must indicate anticoagulant status to avoid delay. A Hematology consult is REQUIRED for inpatient samples (except for patients in Hematology/Oncology ward). Referred-in patients and outpatients can be tested without a Hematology consult.

Write your initials, date and time of collection on the collection label.

Transportation: In/Outpatients: Send whole citrated blood at room temperature as soon as possible. Referred-in: Send frozen citrated plasma on dry ice. All samples must be sent to the Core Laboratory.

Stability: Specimens are stable for 4 hours post collection at room temperature. Specimens which are not tested within 4 hours can be double-spun to remove the citrated plasma. The citrated plasma is stable at -20°C for up to two weeks or -70°C for up to six months.

Sample Rejection Criteria: Clotted sample, grossly hemolyzed samples, Unlabeled or samples with insufficient quantity will be rejected.

Required Documentation

Turnaround Time (TAT)

1 day

Test Utilization

Reference Value

0.00 – 0.60 U/mL

< 1.0 U/mL is negative

≥ 1.0 U/mL is positive

Test Code

HIT

Methodology

Latex Immunoassay

Testing Location

SMH-Special Coagulation

Other Information

Last Updated: June 06, 2019