Laboratory Medicine

Test catalogue

HLA Antibody Screening and/or HLA Typing
Test Name HLA Antibody Screening and/or HLA Typing (HLA Alloantibody Screening)
Specimen Type / Requirements Container Type = 2 Pink Tops, 1 Red Tops Minimum Venous Volume = 2 x 7 ml EDTA specimen (Pink Top) Minimum Venous Volume = 1 x 1 Red Tops Tube must fill to at least ½ or greater of its vacuum capacity
Specimen Handling Collection Instructions: Collect sample in 2 Pink Top tubes and 1 Red Top tube. Samples should be well mixed at time of collection. Requisition must have ordering physician, phlebotomist signature, collection date and time. Specimen must be clearly labeled with patient information. Medical Director Approval required. Stability: Stable at room temperature for 2 hrs Transportation:Send to Transfusion Medicine lab at room temperature as soon as possible. Sample Rejection Criteria: Grossly hemolytic, icteric or lipemic specimens. Unlabeled and/or mislabeled specimens. Incomplete TM requisitions.
Required Documentation Ensure requisition is complete with ordering physician name and phlebotomist signature, date and time of draw.
Turnaround Time (TAT) 3-5 business days	Test Utilization As Required
Reference Value See Interpretive Report
Test Code SENDO	Methodology PCR - Sanger Sequencing; Thermocycler
Testing Location Referred Out – ON, Canada (ROUHNHLA37)	Other Information Last Updated: June 06, 2019

HLA Antibody Screening and/or HLA Typing

Test Name

HLA Antibody Screening and/or HLA Typing (HLA Alloantibody Screening)

Specimen Type / Requirements

Container Type = 2 Pink Tops, 1 Red Tops

Minimum Venous Volume = 2 x 7 ml EDTA specimen (Pink Top)

Minimum Venous Volume = 1 x 1 Red Tops

Tube must fill to at least ½ or greater of its vacuum capacity

Specimen Handling

Collection Instructions: Collect sample in 2 Pink Top tubes and 1 Red Top tube. Samples should be well mixed at time of collection. Requisition must have ordering physician, phlebotomist signature, collection date and time. Specimen must be clearly labeled with patient information. Medical Director Approval required.

Stability: Stable at room temperature for 2 hrs

Transportation:Send to Transfusion Medicine lab at room temperature as soon as possible.

Sample Rejection Criteria: Grossly hemolytic, icteric or lipemic specimens. Unlabeled and/or mislabeled specimens. Incomplete TM requisitions.

Required Documentation

Ensure requisition is complete with ordering physician name and phlebotomist signature, date and time of draw.

Turnaround Time (TAT)

3-5 business days

Test Utilization

As Required

Reference Value

See Interpretive Report

Test Code

SENDO

Methodology

PCR - Sanger Sequencing; Thermocycler

Testing Location

Referred Out – ON, Canada (ROUHNHLA37)

Other Information

Last Updated: June 06, 2019