Laboratory Medicine
Test catalogue
Direct Antiglobulin Test (DAT) | |
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Test NameDirect Antiglobulin Test (DAT, Coombs Test, Direct Coombs) |
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Specimen Type / RequirementsContainer Type = 1 Pink Top Minimum Venous Volume = 1 x 7 ml EDTA specimen (Pink Top) Tube must fill to at least ½ or greater of its vacuum capacity |
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Specimen HandlingCollection Instructions: Collect sample in 1 Pink Top tube. Sample should be well mixed at time of collection. Requisition must have ordering physician, phlebotomist signature, collection date and time. Specimen must be clearly labeled with patient information. EDC required for prenatal patients. Please include diagnosis, transfusion and pregnancy history if applicable. Stability: Stable at room temperature for 8 hrs. Transportation: Send to Transfusion Medicine lab at room temperature as soon as possible. Sample Rejection Criteria: Grossly hemolytic, icteric or lipemic specimens. Unlabeled and/or mislabeled specimens. Incomplete TM requisitions. |
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Required DocumentationEnsure requisition is complete with ordering physician name and phlebotomist signature, date and time of draw. |
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Turnaround Time (TAT)TAT: STAT: 1 hour, Routine: 4-6 hours. TAT may be longer if result is positive |
Test UtilizationAs Required |
Reference ValueNegative |
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Test CodeDAT |
MethodologyHemagglutination |
Testing LocationSMH-Transfusion Medicine (Blood Bank) |
Other InformationLast Updated: October 08, 2019
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