Laboratory Medicine

Test catalogue

B-Hydroxybutyrate (Beta Hydroxybutyrate, BHB)
Test Name B-Hydroxybutyrate (Beta Hydroxybutyrate, BHB, Ketones)
Specimen Type / Requirements Container Type = 1 Gold Top (SST) Minimum Testing Volume = 2mL serum Tube must fill to at least ½ or greater of its vacuum capacity Note: BHB is known to be the single best indicator of ketones in diabetic ketoacidosis, and explains why most laboratories have moved away from serum ketone testing, and directly measure beta-hydroxybutyrate instead.
Specimen Handling Collection Instructions: Collect sample in gold top (SST) tube. Write your initials, date and time of collection on the collection label. Laboratory Staff Instructions: The test is now done in-house. Sperate as soon and possible. Rejection Criteria: Grossly hemolyzed, lipemic, Unlabeled or Not Sufficient Quantity Samples will be rejected. Avoid repeat freeze/Thaw cycles. Stability: Stable refrigrated for 14 days 2-8°C and frozen at or below -20°C for more than 14 days. Transportation: Send sample to core lab as soon as possible.
Required Documentation
Turnaround Time (TAT) 2 hours stat/trauma 4 hours routine.	Test Utilization
Reference Value Pediatric(0-18 years): 0-0.29 mmol/L Adults: 0-0.42 mmol/L >2.0 mmol/L is suggestive of ketoacidosis
Test Code BHB	Methodology Enzymatic
Testing Location SMH-Biochemistry	Other Information Last Updated: Aug 29, 2022

B-Hydroxybutyrate (Beta Hydroxybutyrate, BHB)

Test Name

B-Hydroxybutyrate (Beta Hydroxybutyrate, BHB, Ketones)

Specimen Type / Requirements

Container Type = 1 Gold Top (SST)

Minimum Testing Volume = 2mL serum

Tube must fill to at least ½ or greater of its vacuum capacity

Note: BHB is known to be the single best indicator of ketones in diabetic ketoacidosis,
and explains why most laboratories have moved away from serum ketone testing,
and directly measure beta-hydroxybutyrate instead.

Specimen Handling

Collection Instructions: Collect sample in gold top (SST) tube. Write your initials, date and time of collection on the collection label.

Laboratory Staff Instructions: The test is now done in-house. Sperate as soon and possible.

Rejection Criteria: Grossly hemolyzed, lipemic, Unlabeled or Not Sufficient Quantity Samples will be rejected. Avoid repeat freeze/Thaw cycles.

Stability: Stable refrigrated for 14 days 2-8°C and frozen at or below -20°C for more than 14 days.

Transportation: Send sample to core lab as soon as possible.

Required Documentation

Turnaround Time (TAT)

2 hours stat/trauma 4 hours routine.

Test Utilization

Reference Value

Pediatric(0-18 years): 0-0.29 mmol/L

Adults: 0-0.42 mmol/L

>2.0 mmol/L is suggestive of ketoacidosis

Test Code

BHB

Methodology

Enzymatic

Testing Location

SMH-Biochemistry

Other Information

Last Updated: Aug 29, 2022