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Anti-Tissue Transglutaminase (anti-TTG/IgA , Celiac Screen)

Test Name

Anti-Tissue Transglutaminase (anti-TTG/IgA, Celiac Screen)

Gold Tube Specimen Type / Requirements

Container Type = 1 Gold Top (SST)

Minimum Testing Volume = 1 ml serum

Tube must fill to at least ½ or greater of its vacuum capacity

Specimen Handling

Collection Instructions: Serum or heparinized plasma collected with or without gel separator. Write your initials, date and time of collection on the collection label or requisition.

Special Instructions Sample may be stored at room temperature for no longer than 8 hours. If the assay will not be completed within 8 hours, refrigerate the sample at 2-8˚C. If the assay will not be completed within 48 hours, freeze the sample at -20˚C or lower. Frozen specimens must be mixed well after thawing and prior to testing.

Rejection Criteria: Gross hemolysis, Unlabeled or Not Sufficient Quantity Samples will be rejected.

Stability: Stable for 8h at room temperature, refrigerated 8 days and frozen 1 year (if frozen within 24h)

Transportation: Send sample to core lab as soon as possible.

Required Documentation

Turnaround Time (TAT)

10 days (batched)

Test Utilization

Routine >10 Days

Reference Value

When IgA is < 0.80 g/L. Anti TTG IgG analysis is performed.

Negative < 20 U

Weak positive: 20 – 30 U

Moderate to strong positive: > 30 U

Test Code

AEMA

Methodology

Spectrophotometric (Kinetic rate method)

Testing Location

SMH-Biochemistry

Other Information