Laboratory Medicine

Laboratory Test Catalogue

Amylase Isoenzymes
Test Name Amylase Isoenzymes (Salivary Amylase, Total Amylase, Pancreatic Amylase)
Specimen Type / Requirements Container Type = 1 Gold Top (SST) Minimum Testing Volume = 1mL serum Tube must fill to at least ½ or greater of its vacuum capacity
Specimen Handling Collection Instructions: Collect specimen in gold or red top tube. Write your initials, date and time of collection on the collection label or requisition. Laboratory Staff Instructions: Separate serum from cells within 2 hours of collection and freeze as soon as possible. Note: Salivary amylase is calculated as the difference between the two measured results (salivary amylase = total amylase-pancreatic amylase). Rejection Criteria: Gross hemolysis, unlabeled or samples of non sufficient quantity. Stability: Stable at room temperature for 1 week, refrigerated at 2–8°C for 1 month and frozen at -15°C to -20°C or lower for 6 months. Transportation: Send sample to core lab as soon as possible.
Required Documentation
Turnaround Time (TAT) 15 days (Batched)	Test Utilization
Reference Value Total Amylase: 20-110 U/L Salivary Amylase: 20 – 75 U/L Ratio (0.43 – 0.67) Pancreatic Amylase: 5 – 55 U/L Ratio (0.38 – 0.56)	Pediatric Total Amylase D0 to D14 3-11: U/L D15 to < 13 weeks: 0-26 U/L 13 weeks to < 1 year: 3-58 U/L 1 year to < 19 years: 29-118 U/L
Test Code AMYI	Methodology Spectrophotometric (Enzymatic/Colorimetric)
Testing Location SMH-Biochemistry	Other Information Last Updated: October 24, 2023

Amylase Isoenzymes

Test Name

Amylase Isoenzymes (Salivary Amylase, Total Amylase, Pancreatic Amylase)

Specimen Type / Requirements

Container Type = 1 Gold Top (SST)

Minimum Testing Volume = 1mL serum

Tube must fill to at least ½ or greater of its vacuum capacity

Specimen Handling

Collection Instructions: Collect specimen in gold or red top tube. Write your initials, date and time of collection on the collection label or requisition.

Laboratory Staff Instructions: Separate serum from cells within 2 hours of collection and freeze as soon as possible. Note: Salivary amylase is calculated as the difference between the two measured results (salivary amylase = total amylase-pancreatic amylase).

Rejection Criteria: Gross hemolysis, unlabeled or samples of non sufficient quantity.

Stability: Stable at room temperature for 1 week, refrigerated at 2–8°C for 1 month and frozen at -15°C to -20°C or lower for 6 months.

Transportation: Send sample to core lab as soon as possible.

Required Documentation

Turnaround Time (TAT)

15 days (Batched)

Test Utilization

Reference Value

Total Amylase: 20-110 U/L

Salivary Amylase: 20 – 75 U/L Ratio (0.43 – 0.67)

Pancreatic Amylase: 5 – 55 U/L Ratio (0.38 – 0.56)

Pediatric

Total Amylase

D0 to D14 3-11: U/L

D15 to < 13 weeks: 0-26 U/L

13 weeks to < 1 year: 3-58 U/L

1 year to < 19 years: 29-118 U/L

Test Code

AMYI

Methodology

Spectrophotometric (Enzymatic/Colorimetric)

Testing Location

SMH-Biochemistry

Other Information

Last Updated: October 24, 2023